{"id":43908,"date":"2021-01-29T10:21:47","date_gmt":"2021-01-29T15:21:47","guid":{"rendered":"https:\/\/dev.inrs.ca\/?p=43908"},"modified":"2021-05-21T12:35:56","modified_gmt":"2021-05-21T16:35:56","slug":"imaging-zebrafish-movements-in-3d-to-better-understand-als-disease","status":"publish","type":"post","link":"https:\/\/dev.inrs.ca\/en\/news\/imaging-zebrafish-movements-in-3d-to-better-understand-als-disease\/","title":{"rendered":"Imaging Zebrafish Movements in 3D to Better Understand ALS Disease"},"content":{"rendered":"\n

An interdisciplinary team of the INRS used an innovative imaging technique for a better understanding of motor deficits in Amyotrophic Lateral Sclerosis (ALS).<\/strong><\/p>\n\n\n\n

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The researchers were able to follow the escape behaviour of normal and disease zebrafish models, in 3D. Their results<\/a> have recently been published in Optica, the flagship journal of the Optical Society (OSA). Professor Jinyang Liang<\/a>, expert in ultra-fast imaging and biophotonics, joined an effort with Professor Kessen Patten<\/a>, specialist in genetics and neurodegenerative diseases. The two groups were able to track the position of zebrafish in real time and capture the 3D motion, using a special imaging technique called dispersion-eliminated coded-aperture light field, or DECALF.<\/p>\n\n\n\n

\u201cIt is a unique feature for the analysis of animal behaviours from a neurodevelopment perspective. Otherwise, we would only be able to see the movement in a plane. Losing one dimension can be misleading when studying the movement. You may think zebrafish move one way, but the reality is quite different,\u201d said the expert.<\/p>\n\n\n\n

Their data revealed asymmetrical orientation angles of the left and right fins, indicating drastic changes in direction during the normal zebrafish’ s escape from the stimulus. In contrast, the diseased zebrafish model showed slow responses and limited movement capacity due to motor deficits.<\/p>\n\n\n\n

Conventional light field cameras capture the information not only in x and y, but also the angle at which the light rays are coming from. This way, you can trace them back to focus on where you want. According to Professor Liang, the problem with this technology is the tradeoff. The image can have a high spatial resolution or a high angle resolution, but not both. The solution for this is the coded aperture light field (CALF) imaging, which can be achieved using digital micromirror devices (DMD).<\/p>\n\n\n\n


An Innovative Design<\/strong><\/h2>\n\n\n\n

The DMD acts like a diffraction element and separates the white light into a rainbow. A DMD alone cannot use it with ambient light or sunlight. \u201cYou could always use a single-wavelength light, but it leads to other disadvantages, since the colour of the light may interfere with the nerve system and affect the experiments. For example, red light could make people aggressive, and blue light is also known to affect the mood,\u201d Professor Liang explains.<\/p>\n\n\n\n

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Schematic of the DECALF system.<\/figcaption><\/figure><\/div>\n\n\n\n

To bypass this limitation, the research team used a second DMD to cancel the rainbow induce by the other one. \u201cWe are the first to use this design to manage the colour dispersion within the entire visible spectrum, which allows us to use white light for this experiment,\u201d says Dr. Jingdan Liu, a postdoctoral fellow at INRS and the first author of this paper. <\/p>\n\n\n\n

\u201cDECALF imaging could open up a new avenue for neuroimaging. For example, we could use this system to see neurons\u2019 activity. We could track the emitted light when a neuron \u2018fires\u2019 to know where the neuron is located in the brain and its connectivity.\u201d <\/p>Jinyang Liang, expert in ultra-fast imaging and biophotonics.<\/cite><\/blockquote>\n\n\n\n

\u201cThanks to the work of Professor Liang, we were able to see the macroscopic behaviour of zebrafish with ALS-like symptoms. We could go even further in the study of this disease by looking at the microscopic scale.Using this innovative imaging approach, we could learn about what is happening in the neural system in normal and disease states in a non-invasive manner,\u201d Professor Patten says.<\/p>\n\n\n\n

\u201cIt is the start of a great collaboration, adds Professor Liang. Thanks to the initiative of Ana Tavares, former director of the \u00c9nergie Mat\u00e9riaux T\u00e9l\u00e9communications Research Centre, and Claude Guertin, director of the Armand-Frappier Sant\u00e9 Biotechnologie Research Centre, we had the chance to see what researchers in other fields were doing and discuss interdisciplinary projects like this one.”<\/p>\n\n\n\n


About the Study<\/strong><\/h3>\n\n\n\n

The article \u201cCoded-aperture broadband light field imaging using digital micromirror devices<\/a>\u201d, by Jingdan Liu, Charlotte Zaouter, Xianglei Liu, Shunmoogum A Patten and Jinyang Liang, was published in the February 2021 issue of the journal of Optica. Researchers received financial support from the Natural Sciences and Engineering Research Council of Canada (NSERC), Canada Foundation for Innovation, Fonds de recherche du Qu\u00e9bec – Nature et technologies (FRQNT), and Fonds de Recherche du Qu\u00e9bec – Sant\u00e9 (FRQS). Professor Kessen Patten acknowledges the support from the Anna Sforza Djoukhadjian Research Chair in ALS.<\/p>\n\n\n

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Other articles you might be interested in<\/h4>\n
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\n \"Infinitely\n <\/div>\n
\n Infinitely small, infinitely fast<\/a>\n <\/div>\n <\/div>\n <\/div>\n<\/div>","protected":false},"excerpt":{"rendered":"

An interdisciplinary team of the INRS used an innovative imaging technique for a better understanding of motor deficits in Amyotrophic Lateral Sclerosis (ALS).<\/p>\n","protected":false},"author":392,"featured_media":44096,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[688],"tags":[],"sectors":[679,731],"class_list":["post-43908","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-innover-a-linrs-en","sectors-sante-en","sectors-materiaux-en"],"acf":[],"yoast_head":"\nImaging Zebrafish Movements in 3D to Better Understand ALS Disease | INRS<\/title>\n<meta name=\"description\" content=\"An interdisciplinary team of the INRS used an innovative imaging technique for a better understanding of motor deficits in Amyotrophic Lateral Sclerosis (ALS).\" \/>\n<meta name=\"robots\" content=\"noindex, follow, max-snippet:-1, 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